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Issue 17, 2009
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Synthesis and bioactivity of poly(HPMA)–lysozyme conjugates: the use of novel thiazolidine-2-thione coupling chemistry

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Abstract

A novel thiazolidine-2-thione functionalized chain transfer agent (CTA) was synthesized and used as a reversible addition–fragmentation chain transfer (RAFT) polymerization agent to prepare well-defined poly-N-(2-hydroxypropyl) methacrylamide (PHPMA). The polymer chains had pre-designed molecular weights, narrow polydispersities and were chain-end functionalized. On incubation with protein (lysozyme) under different pH conditions, PHPMA was conjugated to the protein surface via covalent amide bonding. The bioactivity of the lysozyme–PHPMA conjugates was assessed using Micrococcus lysodeikticus (Ml) cells as substrates. The number of polymer chains attached to the protein could be controlled by both the pH of the conjugation reaction and the molecular weights of the polymers, thereby influencing significantly the bioactivity of the proteinpolymer conjugates.

Graphical abstract: Synthesis and bioactivity of poly(HPMA)–lysozyme conjugates: the use of novel thiazolidine-2-thione coupling chemistry

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Publication details

The article was received on 07 Apr 2009, accepted on 08 Jun 2009 and first published on 06 Jul 2009


Article type: Paper
DOI: 10.1039/B907061C
Citation: Org. Biomol. Chem., 2009,7, 3481-3485
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    Synthesis and bioactivity of poly(HPMA)–lysozyme conjugates: the use of novel thiazolidine-2-thione coupling chemistry

    L. Tao, J. Liu, J. Xu and T. P. Davis, Org. Biomol. Chem., 2009, 7, 3481
    DOI: 10.1039/B907061C

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