Issue 13, 2009
From the journal:

Organic & Biomolecular Chemistry

Synthesis and properties of a novel molecular beacon containing a benzene-phosphate backbone at its stem moiety

Yoshihito Ueno,*abce   Akihiro Kawamura,a   Keiji Takasu,a   Shinji Komatsuzaki,a   Takumi Kato,a   Satoru Kuboe,a   Yoshiaki Kitamuraa  and  Yukio Kitade*abcd  

* Corresponding authors

a Department of Biomolecular Science, Faculty of Engineering, Gifu University, Japan
E-mail: uenoy@gifu-u.ac.jp, ykkitade@gifu-u.ac.jp
Fax: +81-58-293-2794
Tel: +81-58-293-2639

b United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Japan

c Center for Emerging Infectious Diseases, Gifu University, Japan

d Center for Advanced Drug Research, Gifu University, 1-1 Yanagido, Gifu, Japan

e PRESTO, JST (Japan Science and Technology Agency), 4-1-8 Honcho Kawaguchi, Saitama, Japan

Abstract

This paper describes the synthesis and properties of a novel molecular beacon (MB) containing a benzene-phosphate backbone at its stem moiety. The fluorescence intensity of MBs was found to stabilize by the introduction of the benzene-phosphate backbone at its stem moiety. Furthermore, an MB containing the benzene-phosphate backbone was more resistant to DNase I (endonuclease) than an MB comprising natural DNA and 2′-O-methyl-RNA. These results indicate that the MB with the benzene-phosphate backbone is superior as a molecular beacon as compared to the MB composed of natural DNA and 2′-O-methyl-RNA.

Graphical abstract: Synthesis and properties of a novel molecular beacon containing a benzene-phosphate backbone at its stem moiety

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Article information

DOI
https://doi.org/10.1039/B901631G
Article type
Paper
Submitted
26 Jan 2009
Accepted
06 Apr 2009
First published
12 May 2009

Org. Biomol. Chem., 2009,7, 2761-2769

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Synthesis and properties of a novel molecular beacon containing a benzene-phosphate backbone at its stem moiety

Y. Ueno, A. Kawamura, K. Takasu, S. Komatsuzaki, T. Kato, S. Kuboe, Y. Kitamura and Y. Kitade, Org. Biomol. Chem., 2009, 7, 2761 DOI: 10.1039/B901631G

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