Issue 4, 2009
From the journal:

Organic & Biomolecular Chemistry

Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences†

Kazuhiro Furukawa,ac   Hiroshi Abe,*a   Jin Wang,a   Miwako Uda,ac   Hiroyuki Koshino,b   Satoshi Tsunedac  and  Yoshihiro Ito*a  

* Corresponding authors

a Nano Medical Engineering Laboratory, Advance Science Institute, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama, Japan
E-mail: h-abe@riken.jp, y-ito@riken.jp

b Molecular Characterization Team Advanced Technology Support Division, Advance Science Institute, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama, Japan

c Department of Life Science and Medical Bio-Science, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo, Japan

Abstract

We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.

Graphical abstract: Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences

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Article information

DOI
https://doi.org/10.1039/B817228E
Article type
Paper
Submitted
01 Oct 2008
Accepted
27 Oct 2008
First published
12 Dec 2008

Org. Biomol. Chem., 2009,7, 671-677

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Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences

K. Furukawa, H. Abe, J. Wang, M. Uda, H. Koshino, S. Tsuneda and Y. Ito, Org. Biomol. Chem., 2009, 7, 671 DOI: 10.1039/B817228E

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