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Issue 22, 2008
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Time-resolved luminescence microscopy of bimetallic lanthanide helicates in living cells

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Abstract

The cellular uptake mechanism and intracellular distribution of emissive lanthanide helicates have been elucidated by time-resolved luminescence microscopy (TRLM). The helicates are non-cytotoxic and taken up by normal (HaCat) and cancer (HeLa, MCF-7) cells by endocytosis and show a late endosomal–lysosomal cellular distribution. The lysosomes predominantly localize around the nucleus and co-localize with the endoplasmatic reticulum. The egress is slow and limited, around 30% after 24 h. The first bright luminescent images can be observed with an external concentration gradient of 5 μM of the EuIII helicate [Q = 0.21, τ = 2.43 ms], compared to >10 μM when using conventional luminescence microscopy. Furthermore, multiplex labeling could be achieved with the TbIII [Q = 0.11, τ = 0.65 ms], and SmIII [Q = 0.0038, τ = 0.030 ms] analogues.

Graphical abstract: Time-resolved luminescence microscopy of bimetallic lanthanide helicates in living cells

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Publication details

The article was received on 04 Jul 2008, accepted on 20 Aug 2008 and first published on 10 Oct 2008


Article type: Paper
DOI: 10.1039/B811427G
Citation: Org. Biomol. Chem., 2008,6, 4125-4133
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    Time-resolved luminescence microscopy of bimetallic lanthanide helicates in living cells

    B. Song, C. D. B. Vandevyver, A. Chauvin and J. G. Bünzli, Org. Biomol. Chem., 2008, 6, 4125
    DOI: 10.1039/B811427G

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