Issue 22, 2007
From the journal:

Organic & Biomolecular Chemistry

Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix†

Gerald Dräger,*a   Csilla Kiss,a   Ulrich Kunzb  and  Andreas Kirschning*a  

* Corresponding authors

a Institut für Organische Chemie and Zentrum für Biomolekulare Wirkstoffchemie (BMWZ), Leibniz Universität Hannover, Schneiderberg 1b, Hannover, Germany
E-mail: draeger@oci.uni-hannover.de
Fax: +49 (0)511-7623011

b Institut für Chemische Verfahrenstechnik der Technischen Universität Clausthal, Leibnizstrasse 17, Clausthal-Zellerfeld, Germany

Abstract

The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly(vinyl benzyl chloride)/poly(vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His6-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6), (R)-2-hydroxy-1-phenylpropan-1-one (7) and 6-O-acetyl-D-glucal (17) in a flow-through mode.

Graphical abstract: Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix

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Article information

DOI
https://doi.org/10.1039/B712804E
Article type
Paper
Submitted
20 Aug 2007
Accepted
18 Sep 2007
First published
05 Oct 2007

Org. Biomol. Chem., 2007,5, 3657-3664

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Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix

G. Dräger, C. Kiss, U. Kunz and A. Kirschning, Org. Biomol. Chem., 2007, 5, 3657 DOI: 10.1039/B712804E

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