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Issue 43, 2015
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Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates

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Abstract

The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface.

Graphical abstract: Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates

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Publication details

The article was received on 17 Aug 2015, accepted on 09 Oct 2015 and first published on 12 Oct 2015


Article type: Communication
DOI: 10.1039/C5NR05427C
Citation: Nanoscale, 2015,7, 18020-18024
  • Open access: Creative Commons BY license
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    Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates

    A. P. Dabkowska, G. Piret, C. S. Niman, M. Lard, H. Linke, T. Nylander and C. N. Prinz, Nanoscale, 2015, 7, 18020
    DOI: 10.1039/C5NR05427C

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