Issue 25, 2015
From the journal:

Nanoscale

Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level†

A. H. Okholm,‡abc   H. Aslan,‡bc   F. Besenbacher,bc   M. Dong*bc  and  J. Kjems*abc  

* Corresponding authors

a Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, 8000 Aarhus C, Denmark
E-mail: kjems@inano.au.dk

b Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark

c Center for DNA Nanotechnology, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark
E-mail: dong@inano.au.dk

Abstract

DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.

Graphical abstract: Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

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Article information

DOI
https://doi.org/10.1039/C5NR01945A
Article type
Communication
Submitted
26 Mar 2015
Accepted
29 May 2015
First published
03 Jun 2015

Nanoscale, 2015,7, 10970-10973

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Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

A. H. Okholm, H. Aslan, F. Besenbacher, M. Dong and J. Kjems, Nanoscale, 2015, 7, 10970 DOI: 10.1039/C5NR01945A

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