A circular dichroism study uncovers a two-step interaction of antitumor azolato-bridged dinuclear platinum(II) complexes with calf thymus DNA

Masako Uemura; Yuko Yoshikawa; Masahiko Chikuma; Seiji Komeda

doi:10.1039/C2MT20022H

A circular dichroism study uncovers a two-step interaction of antitumor azolato-bridged dinuclear platinum(ii) complexes with calf thymus DNA†

Masako Uemura,^a Yuko Yoshikawa,^b Masahiko Chikuma^c and Seiji Komeda*^a

* Corresponding authors

^a Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka, Mie, Japan
E-mail: komedas@suuzka-u.ac.jp
Fax: +81 59 368 1274
Tel: +81 59 340 0581

^b Department of Biotechnology, College of Life Sciences, Ritsumeikan University, Kusatsu, Shiga, Japan
E-mail: yyoshi@fc.ritsumei.ac.jp
Fax: +81 77 561 5064
Tel: +81 77 561 5064

^c Department of Pharmaceutical Sciences, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan
E-mail: chikuma@gly.oups.ac.jp
Fax: +81 72 690 1002
Tel: +8172 690 1004

Abstract

The interactions of four antitumor azolato-bridged dinuclear platinum(II) complexes, [{cis-Pt(NH₃)₂}₂(μ-OH)(μ-azolato)]²⁺, with calf thymus DNA were monitored dose- and time-dependently, by using circular dichroism. Complexes 1–4 reacted with DNA via a two-step interaction that comprised a prompt diffusion-controlled reaction, which induced a B- to C-form transition, and a relatively slow temperature-dependent reaction.

This article is part of the themed collection: Metals and Genetics

Metallomics

A circular dichroism study uncovers a two-step interaction of antitumor azolato-bridged dinuclear platinum(ii) complexes with calf thymus DNA†

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