Facilitating the presentation of antigen peptides on dendritic cells for cancer immunotherapy using a polymer-based synthetic receptor

Cuicui Li; Masafumi Takeo; Masayoshi Matsuda; Hiroko Nagai; Sun Xizheng; Wataru Hatanaka; Akihiro Kishimura; Hiroyuki Inoue; Kenzaburo Tani; Takeshi Mori; Yoshiki Katayama

doi:10.1039/C7MD00188F

Facilitating the presentation of antigen peptides on dendritic cells for cancer immunotherapy using a polymer-based synthetic receptor†‡

Cuicui Li,^a Masafumi Takeo,^a Masayoshi Matsuda,^a Hiroko Nagai,^a Sun Xizheng,^a Wataru Hatanaka,^b Akihiro Kishimura,

^abcd Hiroyuki Inoue,^e Kenzaburo Tani,^e Takeshi Mori

*^abc and Yoshiki Katayama*^abcdfg

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* Corresponding authors

^a Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
E-mail: ykatatcm@mail.cstm.kyushu-u.ac.jp
Tel: +81 092 802 2850

^b Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan

^c Center for Advanced Medical Innovation, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan

^d International Research Center for Molecular Systems, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan

^e Division of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan

^f Center for Advanced Medical Innovation, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan

^g Department of Biomedical Engineering, Chung Yuan Christian University, 200 Chung Pei Road, Chung Li District, Taoyuan 32023, Taiwan

Abstract

The introduction of proteins into dendritic cells (DCs) ex vivo is a critical step for the DC-based immunotherapy of cancer. Here, we developed a biotin-modified polymer with multiple hydrophobic membrane anchors for cells that functions as a synthetic receptor for an antigen protein, ovalbumin (OVA), to introduce it efficiently into DCs compared with the conventional pulsing method. Our method showed significant advantages, including the rapid incorporation of OVA and the activation of antigen-specific T cells in a MHC-restricted manner. When mice were immunized by DCs treated with our method, tumor growth was completely suppressed, indicating that our method can be used to prepare adjuvant DCs.

MedChemComm

Facilitating the presentation of antigen peptides on dendritic cells for cancer immunotherapy using a polymer-based synthetic receptor†‡

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Facilitating the presentation of antigen peptides on dendritic cells for cancer immunotherapy using a polymer-based synthetic receptor

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