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Issue 2, 2015
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Novel β-carboline–quinazolinone hybrid as an inhibitor of Leishmania donovani trypanothione reductase: Synthesis, molecular docking and bioevaluation

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Abstract

Trypanothione reductase (TR) is a vital enzyme in the trypanothione based redox metabolism of trypanosomatid parasites. It is one of the few chemically validated targets for Leishmania. Herein, we report the synthesis of novel β-carboline–quinazolinone hybrids that are able to inhibit Leishmania donovani TR (LdTR) and subsequently inhibit cell growth. A molecular modeling approach based on docking studies and subsequent binding free energy estimation was performed in the active site of LdTR to understand their possible binding sites. With the enzymatic assay on LdTR with compounds, we were able to identify six hit compounds (8j–8o) that were all found to be the competitive inhibitors of TR with Ki in the range of 0.8–9.2 μM. The whole-cell screening assay highlighted the analogues 8k, 8l and 8n as the most active compounds with IC50 of 4.4, 6.0 and 4.3 μM, respectively, along with an adequate selectivity index (SI) of >91, 36 and 24, respectively.

Graphical abstract: Novel β-carboline–quinazolinone hybrid as an inhibitor of Leishmania donovani trypanothione reductase: Synthesis, molecular docking and bioevaluation

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Publication details

The article was received on 08 Jul 2014, accepted on 03 Nov 2014 and first published on 04 Nov 2014


Article type: Concise Article
DOI: 10.1039/C4MD00298A
Author version available: Download Author version (PDF)
Citation: Med. Chem. Commun., 2015,6, 351-356
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    Novel β-carboline–quinazolinone hybrid as an inhibitor of Leishmania donovani trypanothione reductase: Synthesis, molecular docking and bioevaluation

    S. S. Chauhan, S. Pandey, R. Shivahare, K. Ramalingam, S. Krishna, P. Vishwakarma, M. I. Siddiqi, S. Gupta, N. Goyal and P. M. S. Chauhan, Med. Chem. Commun., 2015, 6, 351
    DOI: 10.1039/C4MD00298A

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