Issue 10, 2017

A cross-platform metabolomics workflow for volume-restricted tissue samples: application to an animal model for polycystic kidney disease

Abstract

Metabolic profiling provides an unbiased view of the physiological status of an organism as a “function” of the metabolic composition of a measured sample. Here, we propose a simple LC-MS based workflow for metabolic profiling of volume-restricted samples, namely individual 20 μm-thick histological sections of a mouse kidney. The main idea of this workflow is to re-use the material after an RPLC-MS run, namely using the volume remaining in the vial after injection, and then introducing a phase changing step to enable HILIC-MS analysis. To test the applicability of the workflow and its ability to extract valuable biological information, we applied it to an animal model of polycystic kidney disease (PKD).

Graphical abstract: A cross-platform metabolomics workflow for volume-restricted tissue samples: application to an animal model for polycystic kidney disease

Supplementary files

Article information

Article type
Communication
Submitted
24 Apr 2017
Accepted
24 Jul 2017
First published
02 Aug 2017

Mol. BioSyst., 2017,13, 1940-1945

A cross-platform metabolomics workflow for volume-restricted tissue samples: application to an animal model for polycystic kidney disease

E. Sánchez-López, H. Happé, E. Steenvoorden, A. L. Crego, M. L. Marina, D. J. M. Peters and O. A. Mayboroda, Mol. BioSyst., 2017, 13, 1940 DOI: 10.1039/C7MB00245A

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