Issue 9, 2011

Characterization of AziR, a resistance protein of the DNA cross-linking agent azinomycin B

Abstract

A protein identified from the Streptomyces sahachiroi genome exhibits a protective effect against the DNA alkylator azinomycin B when heterologously expressed in S. lividans and E. coli. The protein, dubbed AziR for azinomycin resistance, is homologous to aminoglycoside phosphotransferases but behaves as an azinomycin binding protein and fails to chemically modify azinomycin. While AziR confers resistance to azinomycin B, it is inactive against aminoglycoside antibiotics and other DNA alkylators. A nucleic acid staining assay indicates that the protein enhances cell survival, and also prevents DNA damage effects normally observed following azinomycin treatment. Knowledge of an azinomycin resistance mechanism aids in setting the stage for future engineered biosynthesis of functionally useful azinomycin analogues.

Graphical abstract: Characterization of AziR, a resistance protein of the DNA cross-linking agent azinomycin B

Supplementary files

Article information

Article type
Paper
Submitted
06 Apr 2011
Accepted
04 Jul 2011
First published
21 Jul 2011

Mol. BioSyst., 2011,7, 2563-2570

Characterization of AziR, a resistance protein of the DNA cross-linking agent azinomycin B

J. Foulke-Abel, G. T. Kelly, H. Zhang and C. M. H. Watanabe, Mol. BioSyst., 2011, 7, 2563 DOI: 10.1039/C1MB05136A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements