Jump to main content
Jump to site search

Issue 7, 2011
Previous Article Next Article

Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis

Author affiliations

Abstract

Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.

Graphical abstract: Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis

Back to tab navigation

Supplementary files

Publication details

The article was received on 03 Feb 2011, accepted on 04 May 2011 and first published on 20 May 2011


Article type: Communication
DOI: 10.1039/C1MB05047H
Citation: Mol. BioSyst., 2011,7, 2114-2117
  •   Request permissions

    Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis

    D. C. Propheter and L. K. Mahal, Mol. BioSyst., 2011, 7, 2114
    DOI: 10.1039/C1MB05047H

Search articles by author

Spotlight

Advertisements