Issue 6, 2011

Glycomics meets lipidomics—associations of N-glycans with classical lipids, glycerophospholipids, and sphingolipids in three European populations

Abstract

Recently, high-throughput technologies have been made available which allow the measurement of a broad spectrum of glycomics and lipidomics parameters in many samples. The aim of this study was to apply these methods and investigate associations between 46 glycan and 183 lipid traits measured in blood of 2041 Europeans from three different local populations (Croatia - VIS cohort; Sweden - NSPHS cohort; Great Britain - ORCADES cohort). N-glycans have been analyzed with High Performance Liquid Chromatography (HPLC) and lipids with Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS) covering sterol lipids, glycerolipids, glycerophospholipids and sphingolipids in eight subclasses. Overall, 8418 associations were calculated using linear mixed effect models adjusted for pedigree, sex, age and multiple testing. We found 330 significant correlations in VIS. Pearson's correlation coefficient r ranged from −0.27 to 0.34 with corresponding p-values between 1.45 × 10−19 and 4.83 × 10−6, indicating statistical significance. A total of 71 correlations in VIS could be replicated in NSPHS (r = [−0.19; 0.35], p = [4.16 × 10−18; 9.38 × 10−5]) and 31 correlations in VIS were also found in ORCADES (r = [−0.20; 0.24], p = [2.69 × 10−10; 7.55 × 10−5]). However, in total only 10 correlations between a subset of triantennary glycans and unsaturated phosphatidylcholine, saturated ceramide, and sphingomyelin lipids in VIS (r = [0.18; 0.34], p = [2.98 × 10−21; 1.69 × 10−06]) could be replicated in both NSPHS and ORCADES. In summary, the results show strong and consistent associations between certain glycans and lipids in all populations, but also population-specific correlations which may be caused by environmental and genetic differences. These associations point towards potential interactive metabolic pathways.

Graphical abstract: Glycomics meets lipidomics—associations of N-glycans with classical lipids, glycerophospholipids, and sphingolipids in three European populations

Supplementary files

Article information

Article type
Paper
Submitted
09 Jul 2010
Accepted
25 Feb 2011
First published
28 Mar 2011

Mol. BioSyst., 2011,7, 1852-1862

Glycomics meets lipidomics—associations of N-glycans with classical lipids, glycerophospholipids, and sphingolipids in three European populations

W. Igl, O. Polašek, O. Gornik, A. Knežević, M. Pučić, M. Novokmet, J. Huffman, C. Gnewuch, G. Liebisch, P. M. Rudd, H. Campbell, J. F. Wilson, I. Rudan, U. Gyllensten, G. Schmitz and G. Lauc, Mol. BioSyst., 2011, 7, 1852 DOI: 10.1039/C0MB00095G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements