Issue 9, 2009
From the journal:

Molecular BioSystems

Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells†‡

Jeffrey K. Takimoto,a   Katrina L. Adams,a   Zheng Xianga  and  Lei Wang*a  

* Corresponding authors

a The Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, USA
E-mail: lwang@salk.edu
Fax: +1 (858) 597-0855
Tel: +1 (858) 453-4100

Abstract

Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.

Graphical abstract: Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells

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Article information

DOI
https://doi.org/10.1039/B904228H
Article type
Communication
Submitted
03 Mar 2009
Accepted
04 Jun 2009
First published
02 Jul 2009

Mol. BioSyst., 2009,5, 931-934

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Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells

J. K. Takimoto, K. L. Adams, Z. Xiang and L. Wang, Mol. BioSyst., 2009, 5, 931 DOI: 10.1039/B904228H

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