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Issue 6, 2008
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A simple strategy for the creation of a recombinant lectin microarray

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Abstract

Glycomics, i.e. the high-throughput analysis of carbohydrates, has yet to reach the level of ease and import of its counterparts, genomics and proteomics, due to the difficulties inherent in carbohydrate analysis. The advent of lectin microarray technology addresses many of these problems, providing a straightforward approach for glycomic analysis. However, current microarrays are limited to the available lectin set, which consists mainly of plant lectins isolated from natural sources. These lectins have inherent problems including inconsistent activity and availability. Also, many plant lectins are glycosylated, complicating glycomic evaluation of complex samples, which may contain carbohydrate-binding proteins. The creation of a recombinant, well-defined lectin set would resolve many of these issues. Herein, we describe an efficient strategy for the systematic creation of recombinant lectins for use in microarray technology. We present a small panel of simple-to-purify bacterially-derived lectins that show reliable activity and define their binding specificities by both carbohydrate microarray and ELISA. We utilize this panel to create a recombinant lectin microarray that is able to distinguish glycopatterns for both proteins and cell samples. This work opens the door to the establishment of a vast set of defined lectinsvia high-throughout approaches, advancing lectin microarray technology for glycomic analysis.

Graphical abstract: A simple strategy for the creation of a recombinant lectin microarray

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Publication details

The article was received on 16 Jan 2008, accepted on 27 Mar 2008 and first published on 14 Apr 2008


Article type: Paper
DOI: 10.1039/B800725J
Citation: Mol. BioSyst., 2008,4, 654-662
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    A simple strategy for the creation of a recombinant lectin microarray

    K. Hsu, J. C. Gildersleeve and L. K. Mahal, Mol. BioSyst., 2008, 4, 654
    DOI: 10.1039/B800725J

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