Issue 3, 2008
From the journal:

Molecular BioSystems

Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity†‡

Wenjuan Zha,§a   Sheryl B. Rubin-Pitela  and  Huimin Zhao*a  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, USA
E-mail: zhao5@uiuc.edu
Fax: (001) 217-333-5052

Abstract

Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescensPf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.

Graphical abstract: Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity

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Article information

DOI
https://doi.org/10.1039/B717705D
Article type
Communication
Submitted
16 Nov 2007
Accepted
16 Jan 2008
First published
31 Jan 2008

Mol. BioSyst., 2008,4, 246-248

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Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity

W. Zha, S. B. Rubin-Pitel and H. Zhao, Mol. BioSyst., 2008, 4, 246 DOI: 10.1039/B717705D

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