Crosslinking of diene-modified DNA with bis-maleimides†
Abstract
The chemical crosslinking of modified nucleic acids via the Diels–Alder reaction is reported. For this purpose, 1,3-butadiene derived building blocks were incorporated into complementary oligodeoxynucleotides. Treatment of the obtained duplex with difunctional dienophiles results in the clean crosslinking of the two strands. Non-crosslinked adducts arising from a single Diels–Alder reaction of a