Issue 23, 2017

A disposable microfluidic device with a reusable magnetophoretic functional substrate for isolation of circulating tumor cells

Abstract

We describe an assembly-disposable microfluidic device based on a silicone-coated release polymer thin film. It consists of a disposable polymeric superstrate and a reusable functional substrate and they are assembled simply using vacuum pressure. The disposable polymeric superstrate is manufactured by bonding a silicone-coated release polymer thin film and a microstructured polydimethylsiloxane (PDMS) replica, containing only a simple structured microchannel. The reusable functional substrate generates an intricate energy field that can penetrate the micrometer-thick polymer film into the microchannel and control microfluids. This is the first report to introduce a silicone-coated release polyethylene terephthalate (PET) thin film as a bonding layer on a microstructured PDMS replica. The bonding strength was ∼600 kPa, which is the strongest among bonding methods of PDMS and PET polymer. Additionally, accelerated tests for bond stability and leakage demonstrated that the silicone-coated release PET film can form a very robust bond with PDMS. To demonstrate the usefulness of the proposed assembly-disposable microfluidic device, a lateral magnetophoretic microseparator was developed in an assembly-disposable microfluidic device format and was evaluated for isolating circulating tumor cells (CTCs) from patients with breast cancer.

Graphical abstract: A disposable microfluidic device with a reusable magnetophoretic functional substrate for isolation of circulating tumor cells

Supplementary files

Article information

Article type
Paper
Submitted
31 Aug 2017
Accepted
27 Oct 2017
First published
27 Oct 2017

Lab Chip, 2017,17, 4113-4123

A disposable microfluidic device with a reusable magnetophoretic functional substrate for isolation of circulating tumor cells

H. Cho, J. Kim, C. Jeon and K. Han, Lab Chip, 2017, 17, 4113 DOI: 10.1039/C7LC00925A

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