Issue 13, 2015
From the journal:

Lab on a Chip

Fast size-determination of intact bacterial plasmids using nanofluidic channels†

K. Frykholm,a   L. K. Nyberg,a   E. Lagerstedt,b   C. Noble,‡b   J. Fritzsche,c   N. Karami,d   T. Ambjörnsson,b   L. Sandegrene  and  F. Westerlund*a  

* Corresponding authors

a Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
E-mail: fredrik.westerlund@chalmers.se

b Department of Astronomy and Theoretical Physics, Lund University, Box 43, SE-221 00 Lund, Sweden

c Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden

d Department of Clinical Microbiology, Sahlgrenska University Hospital and Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden

e Department of Medical Biochemistry and Microbiology, Uppsala University, Box 582, SE-751 23 Uppsala, Sweden

Abstract

We demonstrate how nanofluidic channels can be used as a tool to rapidly determine the number and sizes of plasmids in bacterial isolates. Each step can be automated at low cost, opening up opportunities for general use in microbiology labs.

Graphical abstract: Fast size-determination of intact bacterial plasmids using nanofluidic channels

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Article information

DOI
https://doi.org/10.1039/C5LC00378D
Article type
Communication
Submitted
30 Mar 2015
Accepted
07 May 2015
First published
07 May 2015
This article is Open Access
Creative Commons BY license

Lab Chip, 2015,15, 2739-2743

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Fast size-determination of intact bacterial plasmids using nanofluidic channels

K. Frykholm, L. K. Nyberg, E. Lagerstedt, C. Noble, J. Fritzsche, N. Karami, T. Ambjörnsson, L. Sandegren and F. Westerlund, Lab Chip, 2015, 15, 2739 DOI: 10.1039/C5LC00378D

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