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Issue 2, 2015
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A siphonage flow and thread-based low-cost platform enables quantitative and sensitive assays

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Abstract

For pump-free, material abundant, portable, and easy-to-operate low-cost microfluidics, a siphonage flow microfluidic thread-based analytical device (S-μTAD) platform enabling quantitative and sensitive assays was designed. Renewable and continuous siphonage flow allowed replicate sampling and detection on one channel/device, obviating some possible inconsistencies among channels or devices. Y-shaped channels were fabricated with polyester cotton blend thread, due to its greater chemiluminescent sensitivity in comparison with that of cotton and polyester threads. S-μTAD sensors for glucose and uric acid were fabricated by using oxidase-immobilized cotton thread as the sample arm of the channels. The acceptable reproducibility and high sensitivity, demonstrated by the relative standard deviations of less than 5% in all cases and the detection limits of 4 × 10−8 mol L−1 for hydrogen peroxide, 1 × 10−7 mol L−1 for glucose, and 3 × 10−6 mol L−1 for uric acid, demonstrated the feasibility of the S-μTAD for quantitative assays. Good agreements between S-μTAD/sensor results and hospital results for blood glucose and uric acid assays indicated the capability of S-μTAD/sensors for the analysis of real samples. These findings proved the utility of siphonage for low-cost microfluidics and the suitability of our S-μTAD design for quantitative assays.

Graphical abstract: A siphonage flow and thread-based low-cost platform enables quantitative and sensitive assays

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Publication details

The article was received on 21 Oct 2014, accepted on 05 Nov 2014 and first published on 06 Nov 2014


Article type: Paper
DOI: 10.1039/C4LC01248H
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2015,15, 495-503
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    A siphonage flow and thread-based low-cost platform enables quantitative and sensitive assays

    F. Lu, Q. Mao, R. Wu, S. Zhang, J. Du and J. Lv, Lab Chip, 2015, 15, 495
    DOI: 10.1039/C4LC01248H

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