Jump to main content
Jump to site search

Issue 18, 2014
Previous Article Next Article

Continuous microcarrier-based cell culture in a benchtop microfluidic bioreactor

Author affiliations

Abstract

Microfluidic bioreactors are expected to impact cell therapy and biopharmaceutical production due to their ability to control cellular microenvironments. This work presents a novel approach for continuous cell culture in a microfluidic system. Microcarriers (i.e., microbeads) are used as growth support for anchorage-dependent mammalian cells. This approach eases the manipulation of cells within the system and enables harmless extraction of cells. Moreover, the microbioreactor uses a perfusion function based on the biocompatible integration of a porous membrane to continuously feed the cells. The perfusion rate is optimized through simulations to provide a stable biochemical environment. Thermal management is also addressed to ensure a homogeneous bioreactor temperature. Eventually, incubator-free cell cultures of Drosophila S2 and PC3 cells are achieved over the course of a week using this bioreactor. In future applications, a more efficient alternative to harvesting cells from microcarriers is also anticipated as suggested by our positive results from the microcarrier digestion experiments.

Graphical abstract: Continuous microcarrier-based cell culture in a benchtop microfluidic bioreactor

Back to tab navigation

Supplementary files

Publication details

The article was received on 14 May 2014, accepted on 26 Jun 2014 and first published on 27 Jun 2014


Article type: Paper
DOI: 10.1039/C4LC00570H
Citation: Lab Chip, 2014,14, 3510-3518
  •   Request permissions

    Continuous microcarrier-based cell culture in a benchtop microfluidic bioreactor

    F. Abeille, F. Mittler, P. Obeid, M. Huet, F. Kermarrec, M. E. Dolega, F. Navarro, P. Pouteau, B. Icard, X. Gidrol, V. Agache and N. Picollet-D'hahan, Lab Chip, 2014, 14, 3510
    DOI: 10.1039/C4LC00570H

Search articles by author

Spotlight

Advertisements