Jump to main content
Jump to site search

Issue 15, 2014
Previous Article Next Article

Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution

Author affiliations

Abstract

We report an automated microfluidic-based platform for single cell analysis that allows for cell culture in free solution with the ability to control the cell growth environment. Using this approach, cells are confined by the sole action of gentle fluid flow, thereby enabling non-perturbative analysis of cell growth away from solid boundaries. In addition, the single cell microbioreactor allows for precise and time-dependent control over cell culture media, with the combined ability to observe the dynamics of non-adherent cells over long time scales. As a proof-of-principle demonstration, we used the platform to observe dynamic cell growth, gene expression, and intracellular diffusion of repressor proteins while precisely tuning the cell growth environment. Overall, this microfluidic approach enables the direct observation of cellular dynamics with exquisite control over environmental conditions, which will be useful for quantifying the behaviour of single cells in well-defined media.

Graphical abstract: Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Jan 2014, accepted on 07 May 2014 and first published on 07 May 2014


Article type: Paper
DOI: 10.1039/C4LC00057A
Author version
available:
Download author version (PDF)
Citation: Lab Chip, 2014,14, 2688-2697
  •   Request permissions

    Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution

    E. M. Johnson-Chavarria, U. Agrawal, M. Tanyeri, T. E. Kuhlman and C. M. Schroeder, Lab Chip, 2014, 14, 2688
    DOI: 10.1039/C4LC00057A

Search articles by author

Spotlight

Advertisements