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Issue 8, 2014
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Application of a three-dimensional (3D) particle tracking method to microfluidic particle focusing

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Abstract

In this paper, a defocusing-based three-dimensional (3D) particle tracking method is presented and demonstrated for microfluidic particle focusing applications. Previous work in particle focusing has verified particle position in two dimensions (2D) using micro-streak velocimetry, or confocal and stereoscopic setups for 3D tracking. The results obtained from the methodology presented are compared with the theoretical and previously observed trends, and it is shown that the defocusing technique provides a simple and precise tool for determining the 3D locations of cell-sized particles in microscale flows (Re ≤ 100). Although similar methods exist for micro-particle image velocimetry (μ-PIV) applications, this is the first implementation of this technique for particle focusing applications.

Graphical abstract: Application of a three-dimensional (3D) particle tracking method to microfluidic particle focusing

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Publication details

The article was received on 05 Dec 2013, accepted on 04 Feb 2014 and first published on 05 Feb 2014


Article type: Paper
DOI: 10.1039/C3LC51352A
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2014,14, 1443-1451
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    Application of a three-dimensional (3D) particle tracking method to microfluidic particle focusing

    M. H. Winer, A. Ahmadi and K. C. Cheung, Lab Chip, 2014, 14, 1443
    DOI: 10.1039/C3LC51352A

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