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Issue 4, 2014
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Durable spatiotemporal surveillance of Caenorhabditis elegans response to environmental cues

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Abstract

Animal response to changes in environmental cues is a complex dynamical process that occurs at diverse molecular and cellular levels. To gain a quantitative understanding of such processes, it is desirable to observe many individuals, subjected to repeatable and well defined environmental cues over long time periods. Here we present WormSpa, a microfluidic system where worms are individually confined in optimized chambers. We show that worms in WormSpa are neither stressed nor starved, and in particular exhibit pumping and egg-laying behaviors equivalent to those of freely behaving worms. We demonstrate the applicability of WormSpa for studying stress response and physiological processes. WormSpa is simple to make and easy to operate, and its design is modular, making it straightforward to incorporate available microfluidic technologies. We expect that WormSpa would open novel avenues of research, hitherto impossible or impractical.

Graphical abstract: Durable spatiotemporal surveillance of Caenorhabditis elegans response to environmental cues

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Publication details

The article was received on 18 Sep 2013, accepted on 28 Nov 2013 and first published on 29 Nov 2013


Article type: Paper
DOI: 10.1039/C3LC51061A
Citation: Lab Chip, 2014,14, 764-770
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    Durable spatiotemporal surveillance of Caenorhabditis elegans response to environmental cues

    R. B. Kopito and E. Levine, Lab Chip, 2014, 14, 764
    DOI: 10.1039/C3LC51061A

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