Issue 3, 2014

Measuring single cell mass, volume, and density with dual suspended microchannel resonators

Abstract

Cell size, measured as either volume or mass, is a fundamental indicator of cell state. Far more tightly regulated than size is density, the ratio between mass and volume, which can be used to distinguish between cell populations even when volume and mass appear to remain constant. Here we expand upon a previous method for measuring cell density involving a suspended microchannel resonator (SMR). We introduce a new device, the dual SMR, as a high-precision instrument for measuring single-cell mass, volume, and density using two resonators connected by a serpentine fluidic channel. The dual SMR designs considered herein demonstrate the critical role of channel geometry in ensuring proper mixing and damping of pressure fluctuations in microfluidic systems designed for precision measurement. We use the dual SMR to compare the physical properties of two well-known cancer cell lines: human lung cancer cell H1650 and mouse lymphoblastic leukemia cell line L1210.

Graphical abstract: Measuring single cell mass, volume, and density with dual suspended microchannel resonators

Supplementary files

Article information

Article type
Paper
Submitted
04 Sep 2013
Accepted
15 Nov 2013
First published
02 Dec 2013

Lab Chip, 2014,14, 569-576

Measuring single cell mass, volume, and density with dual suspended microchannel resonators

A. K. Bryan, V. C. Hecht, W. Shen, K. Payer, W. H. Grover and S. R. Manalis, Lab Chip, 2014, 14, 569 DOI: 10.1039/C3LC51022K

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