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Issue 16, 2013
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A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

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Abstract

We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Å via on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.

Graphical abstract: A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

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Publication details

The article was received on 26 Feb 2013, accepted on 12 Jun 2013 and first published on 13 Jun 2013


Article type: Communication
DOI: 10.1039/C3LC50276G
Citation: Lab Chip, 2013,13, 3183-3187
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    A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

    S. L. Perry, S. Guha, A. S. Pawate, A. Bhaskarla, V. Agarwal, S. K. Nair and P. J. A. Kenis, Lab Chip, 2013, 13, 3183
    DOI: 10.1039/C3LC50276G

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