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Issue 9, 2013
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Fast spore breaking by superheating

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Abstract

Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120–180 °C. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 °C resulted in the release of DNA within 20 s.

Graphical abstract: Fast spore breaking by superheating

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Publication details

The article was received on 26 Nov 2012, accepted on 08 Feb 2013 and first published on 11 Feb 2013


Article type: Technical Innovation
DOI: 10.1039/C3LC41305E
Citation: Lab Chip, 2013,13, 1695-1698
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    Fast spore breaking by superheating

    A. Přibylka, A. V. Almeida, M. O. Altmeyer, J. Petr, J. Ševčík, A. Manz and P. Neužil, Lab Chip, 2013, 13, 1695
    DOI: 10.1039/C3LC41305E

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