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Issue 7, 2013
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Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients

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Abstract

An automated microfluidic cell culture platform that creates and maintains independently programmable diffusion-based gradients is reported. Temporal modulation of the source and sink flow patterns allow generation of extremely stable spatial gradients. We developed a system that integrates 30 parallel gradients in a single device, with 10 different chemical formulations and 3 replicates. Mammalian fibroblast and macrophage cells were screened for NFκB pathway activity under gradients of TNFα, PDGF, and LPS, and multiparameter measurements were performed to demonstrate the capability of the device in dynamic single-cell analysis.

Graphical abstract: Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients

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Publication details

The article was received on 23 Sep 2012, accepted on 17 Jan 2013 and first published on 18 Jan 2013


Article type: Paper
DOI: 10.1039/C3LC41076E
Citation: Lab Chip, 2013,13, 1273-1281
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    Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients

    T. Frank and S. Tay, Lab Chip, 2013, 13, 1273
    DOI: 10.1039/C3LC41076E

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