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Issue 1, 2013
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Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes

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Abstract

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.

Graphical abstract: Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes

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Publication details

The article was received on 19 Aug 2012, accepted on 25 Oct 2012 and first published on 26 Oct 2012


Article type: Technical Innovation
DOI: 10.1039/C2LC40948H
Citation: Lab Chip, 2013,13, 68-72
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    Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes

    G. Arrabito, C. Galati, S. Castellano and B. Pignataro, Lab Chip, 2013, 13, 68
    DOI: 10.1039/C2LC40948H

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