Issue 16, 2012

DNA circuits as amplifiers for the detection of nucleic acids on a paperfluidic platform

Abstract

This article describes the use of non-enzymatic nucleic acid circuits based on strand exchange reactions to detect target sequences on a paperfluidic platform. The DNA circuits that were implemented include a non-enzymatic amplifier and transduction to a fluorescent reporter; these yield an order of magnitude improvement in detection of an input nucleic acid signal. To further improve signal amplification and detection, we integrated the enzyme-free amplifier with loop-mediated isothermal amplification (LAMP). By bridging the gap between the low concentrations of LAMP amplicons and the limits of fluorescence detection, the non-enzymatic amplifier allowed us to detect as few as 1200 input templates in a paperfluidic format.

Graphical abstract: DNA circuits as amplifiers for the detection of nucleic acids on a paperfluidic platform

Article information

Article type
Paper
Submitted
18 Apr 2012
Accepted
25 May 2012
First published
30 May 2012

Lab Chip, 2012,12, 2951-2958

DNA circuits as amplifiers for the detection of nucleic acids on a paperfluidic platform

P. B. Allen, S. A. Arshad, B. Li, X. Chen and A. D. Ellington, Lab Chip, 2012, 12, 2951 DOI: 10.1039/C2LC40373K

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