Issue 13, 2012
From the journal:

Lab on a Chip

Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device†

Sewoon Han,‡a   Kisuk Yang,‡b   Yoojin Shin,a   Jung Seung Lee,b   Roger D. Kamm,cd   Seok Chung*a  and  Seung-Woo Cho*b  

* Corresponding authors

a Department of Mechanical Engineering, Korea University, #512B, Innovation Hall, Anam, Seongbuk, Seoul, 136-713, South Korea
E-mail: sidchung@korea.ac.kr
Fax: +82 2 926 9290
Tel: +82 2 3290 3352

b Department of Biotechnology, Yonsei University, #525, 2nd Engineering Hall, 50 Yonsei-ro, Seodaemun, Seoul, 120-749, South Korea
E-mail: seungwoocho@yonsei.ac.kr
Fax: +82 2 362 7265
Tel: +82 2 2123 5662

c Departments of Mechanical Engineering, Cambridge, USA

d Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., NE47-321, Cambridge, MA 02139, USA

Abstract

Here, we report a unique method to quantify the effects of in vivo-like extracellular matrix (ECM) for guiding differentiation of neural stem cells (NSCs) in three-dimensional (3D) microenvironments using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully monitored and quantified differentiation of NSCs in small volume ECMs and found that differentiation of NSCs, especially those differentiating towards neuronal and oligodendrocytic lineages, is significantly enhanced by 3D microenvironments reconstituted in the microfluidic channels.

Graphical abstract: Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device

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Article information

DOI
https://doi.org/10.1039/C2LC21285D
Article type
Communication
Submitted
23 Dec 2011
Accepted
05 Apr 2012
First published
23 May 2012

Lab Chip, 2012,12, 2305-2308

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Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device

S. Han, K. Yang, Y. Shin, J. S. Lee, R. D. Kamm, S. Chung and S. Cho, Lab Chip, 2012, 12, 2305 DOI: 10.1039/C2LC21285D

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