Issue 7, 2012

Optofluidic differential spectroscopy for absorbance detection of sub-nanolitre liquid samples

Abstract

We present a novel optofluidic differential method for carrying out absorbance spectroscopy of sub-nanolitre volumes of liquid samples on a microfluidic chip. Due to the reduction of liquid volume, the absorbance detection in microfluidics is often hindered by either low sensitivity or complex fabrication. To address this issue, we introduced an optofluidic modulator which can be easily integrated into a PDMS (polydimethylsiloxane) based microfluidic chip. The modulator was controlled by the fluid pressure and the absorbance spectrum of the analyte was obtained by taking differential measurements between the analyte and reference medium. An advantage is that this method doesn't need a complicated fabrication step. It is compatible with conventional microfluidic chips and measurements can be carried out on a normal transmission microscope. The performance of the device was tested by measuring solutions containing methylene blue, with concentrations as low as 13 μM.

Graphical abstract: Optofluidic differential spectroscopy for absorbance detection of sub-nanolitre liquid samples

Supplementary files

Article information

Article type
Technical Innovation
Submitted
22 Oct 2011
Accepted
26 Jan 2012
First published
15 Feb 2012

Lab Chip, 2012,12, 1251-1254

Optofluidic differential spectroscopy for absorbance detection of sub-nanolitre liquid samples

W. Song and J. Yang, Lab Chip, 2012, 12, 1251 DOI: 10.1039/C2LC21025H

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