Issue 20, 2011

Rapid detection ofalgal toxins by microfluidic immunoassay

Abstract

Herein we report fabricating a microfluidic device to monitor harmful algal blooming (HAB). The heterogeneous immuno-enzyme assay was integrated into a self-designed microfluidic chip for rapid and automatic analysis of algal toxins. The device was made from polydimethylsiloxane (PDMS) and was assembled with a home-made control system. The performance of the system was demonstrated by the detection of microcystin, saxitoxin and cylindrospermopsin, the major cyanotoxins. In one single microfluidic chip, multiple samples were controlled and analysed in a parallel manner. Under the optimal conditions, the linear range and the limit of detection of microcystins were 0–5.0 ng mL−1 and 0.02 ng mL−1 respectively. The total analysis time was less than 25 min. The designed device was highly automatic, more efficient and economic compared to conventional techniques.

Graphical abstract: Rapid detection ofalgal toxins by microfluidic immunoassay

Additions and corrections

Article information

Article type
Paper
Submitted
14 Jun 2011
Accepted
12 Jul 2011
First published
31 Aug 2011

Lab Chip, 2011,11, 3516-3522

Rapid detection ofalgal toxins by microfluidic immunoassay

J. Zhang, S. Liu, P. Yang and G. Sui, Lab Chip, 2011, 11, 3516 DOI: 10.1039/C1LC20516A

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