Issue 18, 2011

Integrated microfluidic bioprocessor for solid phase capture immunoassays

Abstract

A microfluidic device for solid-phase immunoassays based on microparticle labeling is developed using microvalve-control structures for automated sample processing. Programmable microvalve control in a multilayer structure provides automated sample delivery, adjustable hydrodynamic washing and compatibility with a wide range of substrates. Capture antibodies are derivatized on glass surfaces within the processor using an APTES patterning method, and magnetic microspheres conjugated with a secondary detection antibody are used as labels in a capture-sandwich format. In this microfluidic processor, washing force can be precisely controlled to remove the nonspecifically bound microparticles. Automated microfluidic immunoassays are demonstrated for mouse immunoglobulin (IgG) and human prostate specific antigen (PSA) with limits of detection of 1.8 and 3 pM, respectively. The sample processor architecture is easily parallelized for high-throughput analysis and easily interfaced with various assay substrates.

Graphical abstract: Integrated microfluidic bioprocessor for solid phase capture immunoassays

Article information

Article type
Paper
Submitted
12 May 2011
Accepted
06 Jul 2011
First published
29 Jul 2011

Lab Chip, 2011,11, 3106-3112

Integrated microfluidic bioprocessor for solid phase capture immunoassays

J. Kim, E. C. Jensen, M. Megens, B. Boser and R. A. Mathies, Lab Chip, 2011, 11, 3106 DOI: 10.1039/C1LC20407F

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