Issue 18, 2011
From the journal:

Lab on a Chip

Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network†

Ning Bao,ad   Gayani C. Kodippili,b   Katie M. Giger,b   Velia M. Fowler,c   Philip S. Low*b  and  Chang Lu*a  

* Corresponding authors

a Department of Chemical Engineering, School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, Virginia, USA
E-mail: changlu@vt.edu
Fax: +1-540-231-5022
Tel: +1-540-231-8681

b Department of Chemistry, Purdue University, West Lafayette, Indiana, USA
E-mail: plow@purdue.edu

c Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA

d School of Public Health, Nantong University, Nantong, P. R. China

Abstract

The network of erythrocyte cytoskeletal proteins significantly influences erythrocyte physical and biological properties. Here we show that the kinetics of erythrocyte lysis during exposure to an electric field is sensitively correlated with defects in the cytoskeletal network. Histograms compiled from single-cell electrical lysis data show characteristics of erythrocyte populations that are deficient in a specific cytoskeletal protein, revealing the presence of cell subpopulations.

Graphical abstract: Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network

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Article information

DOI
https://doi.org/10.1039/C1LC20365G
Article type
Communication
Submitted
01 May 2011
Accepted
06 Jul 2011
First published
25 Jul 2011

Lab Chip, 2011,11, 3053-3056

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Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network

N. Bao, G. C. Kodippili, K. M. Giger, V. M. Fowler, P. S. Low and C. Lu, Lab Chip, 2011, 11, 3053 DOI: 10.1039/C1LC20365G

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