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Issue 17, 2011
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Continuous dielectrophoretic bacterial separation and concentration from physiological media of high conductivity

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Abstract

Biological sample processing involves purifying target analytes from various sample matrices and concentrating them to a small volume from a large volume of crude sample. This complex process is the major obstacle for developing a microfluidic diagnostic platform. In this study, we present a microfluidic device that can continuously separate and concentrate pathogenic bacterial cells from complex sample matrices such as cerebrospinal fluid and whole blood. Having overcome critical limitations of dielectrophoretic (DEP) operation in physiological media of high conductivity, we utilized target specific DEP techniques to incorporate cell separation, medium exchange, and target concentration into an integrated platform. The proposed microfluidic device can uptake mL volumes of crude biological sample and selectively concentrate target cells into a submicrolitre volume, providing ∼104 fold of concentration. We designed the device based on the electrokinetic theory and electric field simulation, and tested the device performance with different sample types. The separation efficiency of the device was as high as 97.0% for a bead mixture in TAE buffer and 94.3% and 87.2% for E. coli in human cerebrospinal fluid and blood, respectively. A capture efficiency of 100% was achieved in the concentration chamber. With a relatively simple configuration, the proposed device provides a robust method of continuous sample processing, which can be readily integrated into a fully automated microfluidic diagnostic platform for pathogen detection and quantification.

Graphical abstract: Continuous dielectrophoretic bacterial separation and concentration from physiological media of high conductivity

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Publication details

The article was received on 11 Apr 2011, accepted on 20 Jun 2011 and first published on 21 Jul 2011


Article type: Paper
DOI: 10.1039/C1LC20307J
Citation: Lab Chip, 2011,11, 2893-2900
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    Continuous dielectrophoretic bacterial separation and concentration from physiological media of high conductivity

    S. Park, Y. Zhang, T. Wang and S. Yang, Lab Chip, 2011, 11, 2893
    DOI: 10.1039/C1LC20307J

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