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Issue 16, 2011
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High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes

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Abstract

Spatially defined neuronal networks have great potential to be used in a wide spectrum of neurobiology assays. We present an original technique for the precise and reproducible formation of neuronal networks. A PDMS membrane comprising through-holes aligned with interconnecting microchannels was used during oxygen plasma etching to dry mask a protein rejecting poly(ethylene glycol) (PEG) adlayer. Patterns were faithfully replicated to produce an oxidized interconnected array pattern which supported protein adsorption. Differentiated human SH-SY5Y neuron-like cells adhered to the array nodes with the micron-scale interconnecting tracks guiding neurite outgrowth to produce neuronal connections and establish a network. A 2.0 μm track width was optimal for high-level network formation and node compliance. These spatially standardized neuronal networks were used to analyse the dynamics of acrylamide-induced neurite degeneration and the protective effects of co-treatment with calpeptin or brain derived neurotrophic factor (BDNF).

Graphical abstract: High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes

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Publication details

The article was received on 25 Mar 2011, accepted on 08 Jun 2011, published on 28 Jun 2011 and first published online on 28 Jun 2011


Article type: Paper
DOI: 10.1039/C1LC20257J
Citation: Lab Chip, 2011,11, 2763-2771
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    High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes

    H. Hardelauf, J. Sisnaiske, A. A. Taghipour-Anvari, P. Jacob, E. Drabiniok, U. Marggraf, J. Frimat, J. G. Hengstler, A. Neyer, C. van Thriel and J. West, Lab Chip, 2011, 11, 2763
    DOI: 10.1039/C1LC20257J

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