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Issue 5, 2011
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10 000-fold concentration increase of the biomarker cardiac troponin I in a reducing union microfluidic chip using cationic isotachophoresis

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Abstract

This paper describes the preconcentration of the biomarker cardiac troponin I (cTnI) and a fluorescent protein (R-phycoerythrin) using cationic isotachophoresis (ITP) in a 3.9 cm long poly(methyl methacrylate) (PMMA) microfluidic chip. The microfluidic chip includes a channel with a 5× reduction in depth and a 10× reduction in width. Thus, the overall cross-sectional area decreases by 50× from inlet (anode) to outlet (cathode). The concentration is inversely proportional to the cross-sectional area so that as proteins migrate through the reductions, the concentrations increase proportionally. In addition, the proteins gain additional concentration by ITP. We observe that by performing ITP in a cross-sectional area reducing microfluidic chip we can attain concentration factors greater than 10 000. The starting concentration of cTnI was 2.3 µg mL−1 and the final concentration after ITP concentration in the microfluidic chip was 25.52 ± 1.25 mg mL−1. To the author's knowledge this is the first attempt at concentrating the cardiac biomarker cTnI by ITP. This experimental approach could be coupled to an immunoassay based technique and has the potential to lower limits of detection, increase sensitivity, and quantify different isolated cTnI phosphorylation states.

Graphical abstract: 10 000-fold concentration increase of the biomarker cardiac troponin I in a reducing union microfluidic chip using cationic isotachophoresis

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Publication details

The article was received on 12 Oct 2010, accepted on 14 Dec 2010 and first published on 10 Jan 2011


Article type: Paper
DOI: 10.1039/C0LC00490A
Citation: Lab Chip, 2011,11, 890-898
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    10 000-fold concentration increase of the biomarker cardiac troponin I in a reducing union microfluidic chip using cationic isotachophoresis

    D. Bottenus, T. Z. Jubery, Y. Ouyang, W. Dong, P. Dutta and C. F. Ivory, Lab Chip, 2011, 11, 890
    DOI: 10.1039/C0LC00490A

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