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Issue 2, 2011
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PDMS free-flow electrophoresis chips with integrated partitioning bars for bubble segregation

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Abstract

In this work, a microfluidic free-flow electrophoresis device with a novel approach for preventing gas bubbles from entering the separation area is presented. This is achieved by integrating partitioning bars to reduce the channel depth between electrode channels and separation chamber in order to obtain electrical contact and simultaneously prevent bubbles from entering the separation area. The three-layer sandwich chip features a reusable carrier plate with integrated ports for fluidic connection combined with a softlithographically cast microfluidic PDMS layer and a sealing glass slide. This design allows for a straightforward and rapid chip prototyping process. The performance of the device is demonstrated by free-flow zone electrophoretic separations of fluorescent dye mixtures as well as by the separation of labeled amines and amino acids with separation voltages up to 297 V.

Graphical abstract: PDMS free-flow electrophoresis chips with integrated partitioning bars for bubble segregation

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Publication details

The article was received on 27 Aug 2010, accepted on 15 Oct 2010 and first published on 08 Nov 2010


Article type: Paper
DOI: 10.1039/C0LC00347F
Citation: Lab Chip, 2011,11, 309-314
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    PDMS free-flow electrophoresis chips with integrated partitioning bars for bubble segregation

    S. Köhler, C. Weilbeer, S. Howitz, H. Becker, V. Beushausen and D. Belder, Lab Chip, 2011, 11, 309
    DOI: 10.1039/C0LC00347F

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