High-throughput sample introduction for droplet-based screening with an on-chip integrated sampling probe and slotted-vial array

Abstract

We developed a droplet-based microfluidic screening system with an on-chip sampling probe integrating multi-channels for sample introduction, reagent merging and nanolitre-scale droplet generation, and a slotted-vial array sample presenting system. The present system was applied in protein crystallization conditions screening with an ultra-high sampling throughput up to 6000 h⁻¹ for different samples.