Jump to main content
Jump to site search

Issue 22, 2009
Previous Article Next Article

Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments

Author affiliations

Abstract

We have developed a multi-channel 3D microfluidic cell culture system (multi-channel 3D-µFCCS) with compartmentalized microenvironments for potential application in human drug screening. To this end, the multi-channel 3D-µFCCS was designed for culturing different 3D cellular aggregates simultaneously to mimic multiple organs in the body. Four human cell types (C3A, A549, HK-2 and HPA) were chosen to represent the liver, lung, kidney and the adipose tissue, respectively. Cellular functions were optimized by supplementing the common medium with growth factors. However, TGF-β1 was found to enhance A549 functions but inhibit C3A functions. Therefore, TGF-β1 was specifically controlled-released inside the A549 compartment by means of gelatin microspheres mixed with cells, thus creating a cell-specific microenvironment. The function of A549 cells was enhanced while the functions of C3A, HK-2 and HPA cells were uncompromised, demonstrating the limited cross-talk between cell culture compartments similar to the in vivo situation. Such a multi-channel 3D-µFCCS could be potentially used to supplement or even replace animal models in drug screening.

Graphical abstract: Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments

Back to tab navigation

Supplementary files

Publication details

The article was received on 24 Jul 2009, accepted on 14 Sep 2009 and first published on 08 Oct 2009


Article type: Paper
DOI: 10.1039/B915147H
Citation: Lab Chip, 2009,9, 3185-3192
  •   Request permissions

    Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments

    C. Zhang, Z. Zhao, N. A. Abdul Rahim, D. van Noort and H. Yu, Lab Chip, 2009, 9, 3185
    DOI: 10.1039/B915147H

Search articles by author

Spotlight

Advertisements