Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins

Ling Chen; Zeng-Tai Chen; Jing Wang; Shou-Jun Xiao; Zu-Hong Lu; Zhong-Ze Gu; Lin Kang; Jian Chen; Pei-Heng Wu; Yan-Chun Tang; Jian-Ning Liu

doi:10.1039/B821265A

Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins†

Ling Chen,^a Zeng-Tai Chen,^a Jing Wang,^a Shou-Jun Xiao,*^a Zu-Hong Lu,^b Zhong-Ze Gu,^b Lin Kang,^c Jian Chen,^c Pei-Heng Wu,^c Yan-Chun Tang^d and Jian-Ning Liu*^d

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* Corresponding authors

^a State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, PR China
E-mail: sjxiao@nju.edu.cn
Fax: +86 25 83314502
Tel: +86 25 83621001

^b State Key Laboratory of Bioelectronics, Southeast University, Nanjing, PR China

^c Department of Electronic Science and Engineering, Nanjing University, Nanjing, PR China

^d Institute of Molecular Medicine, Nanjing University, Nanjing, PR China
E-mail: jianningliu2000@yahoo.com

Abstract

A proof-of-concept study demonstrated the feasibility of a novel gel-pad microarray on porous silicon chips, by initiation of an atom transfer radical propagation (ATRP) polymerisation of (polyethylene glycol) methacrylate (PEGMA) with surface Si–H species, stepwise chemical conversions of the gel membrane to an NTA-Ni²⁺/histidine-tagged protein system, and matrix-assisted laser desorption/ionisation mass spectroscopy (MALDI MS) and fluorescence detections.

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Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins†

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Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins

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