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Issue 1, 2009
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Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells

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Abstract

The soft lithographic technique is a collection of simple and cost-effective patterning techniques which applies an elastomeric stamp to transfer a nano/micro-scale pattern. Patterning biological materials using soft lithography provides procedurally simple control of the surface chemistry and the cell environments. However, conventional methods for generating microstructures on a substrate require expensive clean room facilities and skillful training. Here we report a simple and inexpensive clean-room free process using a conventional photomask film as a master to fabricate elastomeric stamps or microfluidic channels. This ultra rapid prototyping technique was applied to print FITC labeled poly-L-lysine with a 10 µm feature size on a glass substrate using soft lithographic processes, such as micro-contact printing and micromolding in capillaries, for patterning human hepatocellular carcinoma cells, human skin fibroblasts and hippocampal neurons from E-18 Sprague-Dawley rat. This novel technique using a photomask film as a master would be very useful ‘hands-on’ tool for the generation of micro-patterned chemical or biological assays using cells and proteins.

Graphical abstract: Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells

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Publication details

The article was received on 18 Jun 2008, accepted on 19 Aug 2008 and first published on 20 Oct 2008


Article type: Technical Note
DOI: 10.1039/B810341K
Citation: Lab Chip, 2009,9, 167-170
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    Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells

    H. Hwang, G. Kang, J. H. Yeon, Y. Nam and J. Park, Lab Chip, 2009, 9, 167
    DOI: 10.1039/B810341K

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