Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 1, 2009
Previous Article Next Article

Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells

Author affiliations

Abstract

The soft lithographic technique is a collection of simple and cost-effective patterning techniques which applies an elastomeric stamp to transfer a nano/micro-scale pattern. Patterning biological materials using soft lithography provides procedurally simple control of the surface chemistry and the cell environments. However, conventional methods for generating microstructures on a substrate require expensive clean room facilities and skillful training. Here we report a simple and inexpensive clean-room free process using a conventional photomask film as a master to fabricate elastomeric stamps or microfluidic channels. This ultra rapid prototyping technique was applied to print FITC labeled poly-L-lysine with a 10 µm feature size on a glass substrate using soft lithographic processes, such as micro-contact printing and micromolding in capillaries, for patterning human hepatocellular carcinoma cells, human skin fibroblasts and hippocampal neurons from E-18 Sprague-Dawley rat. This novel technique using a photomask film as a master would be very useful ‘hands-on’ tool for the generation of micro-patterned chemical or biological assays using cells and proteins.

Graphical abstract: Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 18 Jun 2008, accepted on 19 Aug 2008 and first published on 20 Oct 2008


Article type: Technical Note
DOI: 10.1039/B810341K
Citation: Lab Chip, 2009,9, 167-170
  •   Request permissions

    Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells

    H. Hwang, G. Kang, J. H. Yeon, Y. Nam and J. Park, Lab Chip, 2009, 9, 167
    DOI: 10.1039/B810341K

Search articles by author