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Issue 2, 2009
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In-situ measurement of cellular microenvironments in a microfluidic device

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Abstract

We report on the integration of optical microsensors into a cell culture microchannel device. We demonstrate the possibility of measuring the glucose and oxygen concentrations in the microenvironment of the mammalian cells cultured in a microchannel device. Furthermore, cell proliferation and morphology could be monitored microscopically while these measurements were being made. Through the use of multiple sensors along the length of the microchannel, concentration gradients of various metabolites, such as oxygen, as well as the effects of cell uptake and perfusion rate of growth medium on these gradients could be studied. As such, the system allowed real-time observations of the cells' response to their chemical microenvironment. Our approach allows cell culture and cell assays to be performed simultaneously in an integrated microchannel system with potential applications as a research tool or drug screening method.

Graphical abstract: In-situ measurement of cellular microenvironments in a microfluidic device

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Publication details

The article was received on 23 Apr 2008, accepted on 19 Aug 2008 and first published on 23 Oct 2008


Article type: Paper
DOI: 10.1039/B806907G
Citation: Lab Chip, 2009,9, 257-262
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    In-situ measurement of cellular microenvironments in a microfluidic device

    Z. Lin, T. Cherng-Wen, P. Roy and D. Trau, Lab Chip, 2009, 9, 257
    DOI: 10.1039/B806907G

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