Issue 11, 2008

Novel microchip for in situTEM imaging of living organisms and bio-reactions in aqueous conditions

Abstract

A novel and disposable microchip (K-kit) with SiO2 nano-membranes was developed and used as a specimen kit for in situ imaging of living organisms in an aqueous condition using transmission electron microscopy (TEM) without equipment modification. This K-kit enabled the successful TEM observation of living Escherichia colicells and the tellurite reduction process in Klebsiella pneumoniae. The K. pneumoniae and Saccharomyces cerevisiae can stay alive in K-kit after continuous TEM imaging for up to 14 s and 42 s, respectively. Besides, different tellurite reduction profiles in cells grown in aerobic and anaerobic environments can be clearly revealed. These results demonstrate that the K-kit developed in this paper can be useful for observing living organisms and monitoring biological processesin situ.

Graphical abstract: Novel microchip for in situTEM imaging of living organisms and bio-reactions in aqueous conditions

Supplementary files

Article information

Article type
Paper
Submitted
26 Mar 2008
Accepted
26 Jun 2008
First published
12 Sep 2008

Lab Chip, 2008,8, 1915-1921

Novel microchip for in situTEM imaging of living organisms and bio-reactions in aqueous conditions

K. Liu, C. Wu, Y. Huang, H. Peng, H. Chang, P. Chang, L. Hsu and T. Yew, Lab Chip, 2008, 8, 1915 DOI: 10.1039/B804986F

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