Issue 5, 2008
From the journal:

Lab on a Chip

Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber†

Kwang Ho Cheong,*a   Dong Kee Yi,*b   Jeong-Gun Lee,a   Jong-Myeon Park,a   Min Jun Kim,c   Joshua B. Edeld  and  Christopher Koa  

* Corresponding authors

a Bio & Health Lab, Samsung Advanced Institute of Technology, Giheung, Korea
E-mail: kwangho.cheong@samsung.com
Fax: +82-31-280-8277
Tel: +82-31-280-9475

b College of Bionanotechnology, Division of Bionanotechnology, Gachon Bionano Research Institute, Kyungwon University, Sungnam, Korea
E-mail: vitalis@kyungwon.ac.kr
Fax: +82-31-750-8563
Tel: +82-31-750-8563

c Department of Mechanical Engineering & Mechanics, Drexel University, Philadelphia, PA, USA

d Department of Chemistry & Institute of Biomedical Engineering, Imperial College London, South Kensington, London, UK

Abstract

The optothermal properties of nanoparticles are of interest for biosensors and highly sensitive biochip applications. In this respect, the longitudinal resonance of Au nanorods was used to transform near infrared energy into thermal energy in a microfluidic chip. The resulting heat generated effectively caused pathogen lysis. Consequently the DNA was extracted out of the cell body and transferred to a PCR system. This resulted in the successful demonstration of a one step real-time PCR system for pathogen detection without removal or changing of reagents.

Graphical abstract: Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber

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Article information

DOI
https://doi.org/10.1039/B717382B
Article type
Technical Note
Submitted
09 Nov 2007
Accepted
25 Feb 2008
First published
11 Mar 2008

Lab Chip, 2008,8, 810-813

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Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber

K. H. Cheong, D. K. Yi, J. Lee, J. Park, M. J. Kim, J. B. Edel and C. Ko, Lab Chip, 2008, 8, 810 DOI: 10.1039/B717382B

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