Micro pumping with cardiomyocyte–polymer hybrid

Abstract

This paper presents a hybrid micropump actuated by the up–down motion of a dome shaped cell-polymer membrane composite. The contractile force induced from self-beating cardiomyocytes cultured on the membrane causes shrinkage and relaxation of a microchamber, leading to a flow in a microchannel. Flow direction is controlled by the geometry of diffuser/nozzle in the microchannel. The fabrication process is noninvasive to cells, thus, cardiomyocytes can robustly maintain their activity for a long time. The fluid motion in the microchannel was monitored by tracking 2 µm polystyrene beads. A net flow rate of 0.226 nl min⁻¹ was obtained in our microscale device. Our device demonstrates a unique performance of a cell–microdevice hybrid lab-on-a-chip that does not require any external power source, preventing electrical or heat shock to analytes.