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Issue 6, 2007
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Micro-bioreactor array for controlling cellular microenvironments

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Abstract

High throughput experiments can be used to spatially and temporally investigate the many factors that regulate cell differentiation. We have developed a micro-bioreactor array (MBA) that is fabricated using soft lithography and contains twelve independent micro-bioreactors perfused with culture medium. The MBA enables cultivation of cells that are either attached to substrates or encapsulated in hydrogels, at variable levels of hydrodynamic shear, and with automated image analysis of the expression of cell differentiation markers. The flow and mass transport in the MBA were characterized by computational fluid dynamic (CFD) modeling. The representative MBA configurations were validated using the C2C12 cell line, primary rat cardiac myocytes and human embryonic stem cells (hESCs) (lines H09 and H13). To illustrate the utility of the MBA for controlled studies of hESCs, we established correlations between the expression of smooth muscle actin and cell density for three different flow configurations.

Graphical abstract: Micro-bioreactor array for controlling cellular microenvironments

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Publication details

The article was received on 03 Jan 2007, accepted on 24 Apr 2007 and first published on 15 May 2007


Article type: Paper
DOI: 10.1039/B700063D
Citation: Lab Chip, 2007,7, 710-719
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    Micro-bioreactor array for controlling cellular microenvironments

    E. Figallo, C. Cannizzaro, S. Gerecht, J. A. Burdick, R. Langer, N. Elvassore and G. Vunjak-Novakovic, Lab Chip, 2007, 7, 710
    DOI: 10.1039/B700063D

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