Issue 8, 2006

Multiplexed analyte and oligonucleotide detection on microarrays using several redox enzymes in conjunction with electrochemical detection

Abstract

We show that multiple enzyme tags may be used in an immunoassay format or for the detection of sequence-specific DNA on microarrays. The assays may be multiplexed and monitored under separate solution and voltage differences. Thus, the detection method relies on an electrochemical detection format, whereby multiple enzymes can be sensed. In our case we utilize horseradish peroxidase, laccase, and glucose dehydrogenase as enzymes attached to specific antibodies or to streptavidin.

Graphical abstract: Multiplexed analyte and oligonucleotide detection on microarrays using several redox enzymes in conjunction with electrochemical detection

Article information

Article type
Paper
Submitted
05 Jan 2006
Accepted
31 May 2006
First published
19 Jun 2006

Lab Chip, 2006,6, 1052-1055

Multiplexed analyte and oligonucleotide detection on microarrays using several redox enzymes in conjunction with electrochemical detection

K. Dill, A. Ghindilis and K. Schwarzkopf, Lab Chip, 2006, 6, 1052 DOI: 10.1039/B600126B

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